Research Techniques

(You gotta have a gimmick if you wanna get ahead*)

Awake, behaving rodents, as well as anesthetized preparations, are utilized in support of our ongoing research. Our technical expertise includes: reinforcement learning; intracellular recording; in vivo multi-site extracellular recording; in vivo juxtacellular recording and staining; intracortical electrical, pharmacological, and optical (optogenetic) microstimulation; intrinsic optical signal imaging; in vivo two-photon laser scanning microscopy, including adaptive optics for deep imaging; transcranial imaging (PoRTS window - shown below); second and third harmonic imaging; in vivo plasmas mediated ablation of tissue; viral transport and expression; classical and viral track tracing, including transsynaptic tracing; high-throughput histology and immunochemistry; automated spike sorting; computational anatomy; analytical and numerical methods for data analysis; machine learning; and single cell and network modeling.

Technical expertise that is no longer in current practice in the labortory involves cortical and brainstem slice physiology; G-protein receptor-based reporters of neuronal signaling (CNiFERs); cell culture, patterned substrates, and in vitro neuronal circuits; and laser light scattering.

We maintain a model shop for projects that involve a mixture of electronic, optical, fluidic, and mechanical design and fabrication.

Research Techniques

Our early research program utilized in vitro techniques with mammalian cell culture, including chemically patterned suibstrates for differential cell adhesion, and invertebrate cell culture uisng isolated neurons from juvenile Aplysia (shown below).

Research Techniques

*Stephen Sondheim (b1930)